Journal: Current protocols

Article Title: Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.

doi: 10.1002/cpz1.875

Figure Lengend Snippet: Figure 1 An illustration of the trophoblast differentiation from PSC leading to formation of trophectoderm (TE)- like cells, further adaptation to TSC medium for complete conversion to PSC-TSC, and terminal differentiation to STB and EVT with each step showing corresponding protocol numbers.

Article Snippet: STB differentiation medium Terminal differentiation medium (see recipe and Table 7) Y-27632, 10 mM (Selleck Chemicals, cat. no. S1049) Knockout serum replacement (KSR) (ThermoFisher, cat. no. 10828028) Forskolin, 10 mM (see recipe) Prepare STB differentiation media fresh prior to feeding.

Techniques:

Journal: Current protocols

Article Title: Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.

doi: 10.1002/cpz1.875

Figure Lengend Snippet: Figure 4 (A) Representative bright phase images (20×) of daily changes during STB differentiation. The confluence should be ∼30% on day 0 to initiate STB differentiation. Scale bar: 100 μm. (B) An illustration of the STB differentiation protocol starting with PSC-TSC. Changes in matrices and media are noted and highlighted with different colors. Arrowheads correspond to time-points requiring media change. (C) Representative hCG ELISA data measured on day 6 STB, compared to the starting TSC state (on day 0). *p < .05, student’s t-test. (D) Representative immunofluorescence image of day 6 STB with cell membrane marker ZO-1 (red). Perforated white line outlines areas with cell fusion. Appropriate cell plating density is necessary for measuring cell-cell fusion. Scale bar 100 μm.

Article Snippet: STB differentiation medium Terminal differentiation medium (see recipe and Table 7) Y-27632, 10 mM (Selleck Chemicals, cat. no. S1049) Knockout serum replacement (KSR) (ThermoFisher, cat. no. 10828028) Forskolin, 10 mM (see recipe) Prepare STB differentiation media fresh prior to feeding.

Techniques: Enzyme-linked Immunosorbent Assay, Membrane, Marker

Journal: Current protocols

Article Title: Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.

doi: 10.1002/cpz1.875

Figure Lengend Snippet: Figure 6 (A) An illustration of the original two-step trophoblast differentiation from PSC used for Horii et al. (2016). (B) An illustration of the improved two-step trophoblast differentiation with addition of IWP2 in the first step differentiation, and change in oxygen tension at the second step differentiation, directing to either EVT lineage by 2% O2 or STB lineage by 21% O2.(C) An illustration of the trophoblast differentiation protocol applying Okae’s STB and EVT differentiation protocol to the TE-like cells.

Article Snippet: STB differentiation medium Terminal differentiation medium (see recipe and Table 7) Y-27632, 10 mM (Selleck Chemicals, cat. no. S1049) Knockout serum replacement (KSR) (ThermoFisher, cat. no. 10828028) Forskolin, 10 mM (see recipe) Prepare STB differentiation media fresh prior to feeding.

Techniques:

Journal: Frontiers in Nutrition

Article Title: Tissue specific role of ABCA1 in lung cholesterol homeostasis under high-cholesterol diet

doi: 10.3389/fnut.2025.1649407

Figure Lengend Snippet: Subcellular localization of ABCA1 in lung tissue. (A) Co-staining of ABCA1 (red) and SFTPC (green, alveolar type II marker); nuclei stained with DAPI (blue). (B) Co-staining of ABCA1 (red) and CD68 (green, macrophage marker) to determine cell-type-specific expression. n = 3–5 mice/group.

Article Snippet: Finally, DAPI was applied in the dark for 5 min, and the sections were sealed with an anti-fade mounting medium CD68 (1:100, boster), SFTPC (1:100, Proteintech), ABCA1(1:100, Proteintech).

Techniques: Staining, Marker, Expressing